This study was conducted to compare the endocrine milieu, and pregnancy rates in In Vitro Fertilization and Embryo Transfer (IVF-ET) program employing combined with gonadotropin releasing hormone agonist (GnRH-a) and pergonal (LH 75IU+FSH 75IU)
when
either human chorionic gonadotropin (HCG) or progesterone were used for luteal phase support.
A total number of 40 IVF-ET treatment cycles were prospectively studied. Ovarian hyperstimulation method was modified ultrashort protocol using GnRH-a. All patients started Decapeptyl at menstrual cycle day #2, and HMG was started at #3 days.
When
leading follicle was ≥18mm or at least two follicles were ≥14mm in diameter, HCG 10000IU intramuscularly was injected. After 36 hours HCG administration, oocytes were retrieved as usual guided by transvaginal ultrasound. Embryo were transfered
36-48
hours later.
The patient's cycles were prospectively randomized to receive HCG (20cycles) or Progesterone (20cycles) for luteal support. The progesterone group received 25mg IM starting from the day of ET. The HCG group received 1500IU IM on days 0, +2, +5
after
ET.
Estadiol (E2) and Progesterone (P4) were measured on the day of oocyte aspiration, ET day, and every 6 days thereafter.
@ES Results were follows as;
@EN 1. Estradiol, progesterone and LH levels on the day of HCG trigger, retrieved oocytes and number of transfered embryos were not significantly different in both groups.
2. On the day of aspiration and embryo transfered day, E2, P4 level were significantly higher in progesterone group than HCG group (p<0.01).
3. E2, P4 level on 6 days after ET were significantly higher in progesterone group than HCG group (p<0.01). But, P4/E2 ratio was not different in both groups.
4. E2, P4 level 12 days after ET were decreased abruptly in both groups and higher hormonal level appeared in HCG group (P<0.01).
5. The total pregnancy rate in the HCG group was 40% (8/20) and in the progesterone group 15% (3/20).
6. Comparing the pregnant and nonpregnant cases progesterone group was not different the hormonal status. In HCG group, pregnant cases appeared in higher P4, P4/E2 ratio than nonpregnanct cases (P<0.01).
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